Tetrahymena genome sequence An example in which we did not obtain a Mar 31, 2025 · Deraniyagala AS, Maier W, Parra M, Nanista E, Sowunmi DO, Hassan M, Chasen N, Sharma S, Lechtreck KF, Cole ES, Bernardes N, Chook YM, Gaertig J (2025) Importin-9 and a TPR domain protein MpH drive periodic patterning of ciliary arrays in Tetrahymena. e. The goals of the project were to generate 8x coverage of the macronuclear genome, assemble the genome sequences, and create databases for the sequence and annotation. (a) IES elimination. May 31, 2011 · Background Despite the fact that the organization of the ciliate mitochondrial genome is exceptional, only few ciliate mitochondrial genomes have been sequenced until today. thermophila MAC genome consists of approximately 180 chromosomes (Hamilton, Dear and Orias, in preparation). Sequences chosen near the ends of the shotgun sequence scaffolds have been used as markers and tested on a HAPPY mapping panel. (2005). The ~120 Mb genome has ~27,500 predicted open reading frames, but is transcriptionally silent during vegetative growth. , 1994, Shang et al. , 1995, Duharcourt et al. The raw sequence data have been deposited in the Genome Sequence Archive in National Genomics Data Center, China National Center for Bioinformation under accession number CRA003720 Jul 16, 2015 · The draft MIC genome sequence (version 2) was obtained from the Tetrahymena Comparative Sequencing Project (Broad Institute of Harvard and MIT). Genome sequences, transcriptomic data, gene models, functional annotation, ortholog groups and synteny maps were built into this Aug 1, 2006 · To address whether a canonical SWI/SNF complex exists in Tetrahymena, we identified putative members by querying the Tetrahymena genome database with sequences encoding subunits of the yeast SWI/SNF complex that have human homologues. thermophila is described, validated and used to study gene expression during the three major Jan 1, 2021 · The complete genome sequences and gene annotation files of T. The Tetrahymena genome sequence can also contribute valuable insights in other areas, including evolution of the genetic code (UAR, along with CAR, are glutamine codons in Tetrahymena) and evolution of immunoglobulin-like DNA rearrangements that occur during MAC differentiation. Expanding our understanding of basic biological processes relevant to human health. , tetra, Gr. TGD Wiki provides information on the genome, genes, and proteins of Tetrahymena collected from scientific literature, the research community, and many other sources. Journal: PLoS Biol 4:e286 (2006) Mar 24, 2000 · In the accompanying paper (Burger et al. , hymen, L. Feb 13, 2012 · Introduction. The MAC genome consists of completely sequenced 180 non-rDNA coding mini-chromosomes, each comprising approximately 90 copies (Sheng et al. The T. thermophila macronuclear genome sequence analysis 3. The predicted IESs range Jan 2, 2020 · Tetrahymena rostrata is a ciliated protist which can parasitize the gray field slug, Deroceras reticulatum. 2008 annotation of the Tetrahymena genome sequence. , in pure culture. Apr 1, 2023 · Secondly, it is very difficult to separate germline from somatic genome and then obtain sufficient quantity of germline DNA for high-throughput genome sequencing (Duan et al. edu. During a detailed comparison of this sequence with that of another ciliate, Paramecium aurelia , we noted a number of unusual structural features that raised questions about mitochondrial Apr 1, 2003 · The complete sequence of the mitochondrial genome of Tetrahymena thermophila has been determined and compared with the mitochondrial genome of Tetrahymena pyriformis. During DNA elimination, IES regions are heterochromatinized and assembled into heterochromatin bodies in the developing somatic nucleus. ucsb. 1. , 2010 , Schoeberl 2. The recent sequencing of the macronucleus genome should ensure that Tetrahymena will be continuously used as a model system. , using multicellular animal models. The first record of Colpidium colpoda was in 1829 by Mathaeo Losana, who placed it in the genus Paramaecia. With the near-complete MIC genome sequence, the predicted number of IESs has been substantially increased and the latest estimation is approximately 12 000 , which corresponds to approximately 46 Mb. , 2002). = four) oral structures – the paroral or undulating membrane and three oral polykinetids or membranelles, which are “membrane”-like (i. Significance of a Tetrahymena Genome Sequencing Project. Jul 1, 2011 · Tetrahymena genomic resources, including genome sequence, transcriptome expression profiles, subcellular organelle proteomes and genetic maps, are listed and referenced as Supplementary Material. , 2010 , Schoeberl Why work with Tetrahymena? Robust methods for strain storage, gene targeting and designer genetic crosses have been developed, complementing the long-standing ease of cytology, micromanipulation and biochemistry. The raw sequence data have been deposited in the Genome Sequence Archive in National Genomics Data Center, China National Center for Bioinformation under accession number CRA003720 and throughput sequencing effort to investigate ciliate genome restructuring, comparing Sanger long-read sequences from a Tetrahymena thermophila MIC genome library to the MAC genome assembly. ***** Tetrahymena's versatile biology provides extremely useful experimental tools. Unicell, complex biology Part of what makes Tetrahymena an excellent model organism is that it possesses the advantages of other laboratory single cell models, yet, it shares with us (multicellular animals) many The complete sequence of the mitochondrial genome of Tetrahymena thermophila has been determined and compared with the mitochondrial genome of Tetrahymena pyriformis. Scanning electron micrograph of a Tetrahymena cell. Tetrahymena is a fresh-water protozoan that is highly successful ecologically. 15: R317-8. Tetrahymena is a microbial eukaryote whose genome sequence would be of value to biomedical researchers and to the entire scientific community for the following reasons: 1) Important microbial eukaryote for biomedical research not approachable in yeast. an essential companion to the ongoing Tetrahymena and Paramecium genome sequences. Jan 1, 2012 · Meanwhile, to make these data more accessible to the community, the Tetrahymena Genome Database (TGD) was established at Stanford University (Stover et al. To characterize the sequence more stringently, we perform a reciprocal best BLAST. BETHESDA, Md. The diploid MIC genome consists of five pairs of chromosomes, whereas the MAC genome Coordinator, Tetrahymena Genome Project Department of Molecular, Cellular and Developmental Biology University of California at Santa Barbara. The Tetrahymena genome sequence is considered to be valuable for fundamental and biomedical research for several reasons: Tetrahymena's history of major discoveries in molecular and cell biology, its important phylogenetic position among eukaryotes, the complexity of its eukaryotic biology, the high degree of functional conservation with the Oct 2, 2023 · Here we completed the first chromosome-level Tetrahymena macronuclear genome assembly, with approximately 300× long Single Molecule, Real-Time reads of the wild-type SB210 cells—the reference . Why Sequence the Tetrahymena Genome: Tetrahymena as a valuable genetic unicellular animal model organism . Santa Barbara, CA 93106 Phone: 805 893 3024 - FAX: 805 893 4724 E-mail: mailto:orias@lifesci. shanghaiensis can be accessed from Tetrahymena Comparative Genomics Database . A. and Gorovsky, M. lscf. Like other ciliates, it shows nuclear dimorphism with a micronucleus (MIC) and a macronucleus (MAC) representing the germline and the somatic functions, respectively. Funding to pay the Open Access publication charges for this article was provided by Jan 1, 2006 · TGD is part of the Tetrahymena Macronuclear Genome Sequencing Project and is funded by NIGMS Grant 5R01GM067012-03 to Jonathan Eisen at TIGR. , 2020, Zhou et al. thermophila and Jan 1, 2012 · Tetrahymena has been assigned without controversy to the family Tetrahymenidae in the hymenostome grouping of ciliates (Lynn, 2008). We will maintain a link on the gene pages to the v. Nov 1, 2008 · Our current understanding of Tetrahymena genome differentiation indicates that the underlying mechanisms by which this occurs appear to be evolved from those that eukaryotes generally use to identify and repress the activity of repetitive sequences and transposon elements. In recent years, single-cell genome sequencing techniques provide an excellent opportunity to explore genome architectures with trace amounts of DNA. All 181 chromosomes were capped with two telomeres and gaps were entirely closed. We have received an update of the Tetrahymena thermophila genome sequence from researchers at Ocean University of China. 7 % A+T) excluding telomeric sequences (identical tandem repeats of 31 bp at each end of the genome). Introduction: Why sequence the Tetrahymena genome?. thermophila EST sequenced from both ends showed that the fusion gene is expressed in this species in vivo. The genome of the ciliated protozoan Tetrahymena thermophila was sequenced at The Institute for Genomic Research (TIGR) in 2003 (). Feb 10, 2009 · Background The model eukaryote, Tetrahymena thermophila, is the first ciliated protozoan whose genome has been sequenced, enabling genome-wide analysis of gene expression. 2021). - The National Human Genome Research Institute (NHGRI) has prioritized the next group of organisms to be considered for entry into the sequencing pipeline as the current efforts with human, mouse and rat approach completion. The raw sequence data have been deposited in the Genome Sequence Archive in National Genomics Data Center, China National Center for Bioinformation under accession number CRA003720 Mar 31, 2025 · Tetrahymena thermophila macronuclear genome fully completed . 1) (see Chapter 5 for more details on DNA elimination in Tetrahymena thermophila removes approximately 12 000 internal eliminated sequences (IESs), which correspond to one-third of the genome, when the somatic macronucleus (MAC) differentiates from the germline micronucleus (MIC). pyriformis mtDNA is a linear molecule of 47,172 bp (78. Like other ciliates, this species has separate germline and soma functions that are embodied by distinct nuclei within a single cell. Nov 3, 2020 · The complete genome sequences and gene annotation files of T. , 1990) into about 200 MAC chromosomes (Eisen et al. In the widely studied model organism, Tetrahymena thermophila, the five MIC chromosomes are fragmented at sites of the 15 bp Chromosome breakage sequence (Cbs) (Yao et al. All ciliate mitochondrial genomes are linear. Such low repetitiveness makes complete closure of the MAC genome a feasible goal, which to achieve would require standard closure methods as well as removal of minor MIC NHGRI Prioritizes Next Organisms to Sequence First group includes chicken, chimpanzee and the honey bee May 2002. Nov 26, 2008 · Background Tetrahymena thermophila, a widely studied model for cellular and molecular biology, is a binucleated single-celled organism with a germline micronucleus (MIC) and somatic macronucleus (MAC). If the Tetrahymena sequence and the query sequence (or other known sequences of that dynein subunit) are best BLAST results for each other, then this is strong evidence that the Tetrahymena sequence is a true ortholog. A part of the micronuclear chromosome (IES, green) is eliminated (dotted lines) and macronucleus destined sequences (MDSs, black) are re-ligated. Concept Papers - White paper submitted to the National Human Genome Research Iinstitue, 2/10/2002 - Concept paper submitted to the Trans-NIH NonMammalian Models Committee, 11/5/2001: Main text, Appendix and References No free-living a lveolate genome has been sequenced. The Tetrahymena genome sequence is considered to be valuable for fundamental and biomedical research for several reasons: Tetrahymena's history of major discoveries in molecular and cell biology, its important phylogenetic position among eukaryotes, the complexity of its eukaryotic biology, the high degree of functional conservation with the genome sequence and provide critical insights into the remarkable genome reorganization processes of ciliates and several diverse adaptations found in the comparison species. neo2 is a chimeric gene that has the 5′ flanking sequence (including the promoter and 5′UTR) from the Tetrahymena histone H4 gene HHF1 and the 3′ flanking sequence (including 3′UTR and probable poly A signal) from the Tetrahymena beta Colpidium colpoda illustration by Alfred Kahl. Dec 12, 2014 · DNA renaturation kinetics studies of the distantly related Tetrahymena soon followed , revealing a 10 to 20% reduction in sequence complexity in the somatic genome. Mar 31, 2025 · Tetrahymena thermophila macronuclear genome fully completed . reticulatum. Future studies of this seemingly unconventional biology will contribute Research in Microbiology, 2011. May 10, 2005 · Ciliates have two types of nucleus with distinct functions. Jan 1, 2012 · The somatic genome of the ciliated protist Tetrahymena undergoes DNA elimination of defined sequences called internal eliminated sequences (IESs), which account for ∼30% of the germline genome. thermophila, which is approximately 104 Mb in length and composed of approximately 225 chromosomes. Tetrahymena thermophila is a ciliate belonging to the order Hymenostomatida within the unicellular eukaryotic organisms (protists). Their unusual Sep 25, 2019 · Ciliates are a large and diverse group of unicellular organisms. In Tetrahymena, the diploid micronucleus is the germline. (2006) Macronuclear Genome Sequence of the Ciliate Tetrahymena thermophila, a Model Eukaryote. We explored polymorphisms at the junctions of programmed eliminated sequences, and reveal their utility to barcode very closely related cells. Jul 16, 2015 · The draft MIC genome sequence (version 2) was obtained from the Tetrahymena Comparative Sequencing Project (Broad Institute of Harvard and MIT). Many observations suggest the potential benefits of the Tetrahymena genome sequence for investigating human neurobiology. Experimental evolution has provided novel insight into a wide array of biological processes. This allowed us to generate comprehensive and robust genetic and physical maps of the germline and somatic The genome sequence and gene models of Tetrahymena thermophila were not determined by the Joint Genome Institute (JGI), but were downloaded from NCBI on May 31, 2018. Here, we report the sequence of the mitochondrial genome (mt genome) of a strain of T As part of the sequencing and analysis of the first ciliate genome, we performed an initial analysis of the Tetrahymena thermophila kinome. , 1987). A Eisen et al. Tetrahymena Genome Database: taxonomy/phylogenetic: NCBI taxonomy bookmarks: ciliate (genome proposal) taxonomy/phylogenetic: NHGRI genome proposal white papers: WebScipio: Tetrahymena thermophila: organism-specific: WebScipio - eukaryotic gene identification: diArk: Tetrahymena thermophila: organism-specific: diArk - a resource for eukaryotic NHGRI Prioritizes Next Organisms to Sequence First group includes chicken, chimpanzee and the honey bee May 2002. Jan 25, 2014 · A puromycin-N-acetyltransferase gene (pac) is widely used as a selection marker for eukaryotic gene manipulation. Species in the genus Tetrahymena are proving to be a highly useful system for studying a range of questions using experimental evolution. Tetrahymena comparative genome database cotain genome information and comparative genomics analysis results for 10 species in the Tetrahymena genus. The genus is named for its four (i. Over the years Tetrahymena has been a leading model organism for the study of telomeres, ribozymes, heterochromatin formation, transcription regulation, cilia and cellular motility, and a host of other basic biological processes (). Aug 29, 2006 · Introduction. Here, we present a complete sequence assembly of the germline genome and analyze Dec 9, 2019 · Tetrahymena rostrata is a ciliated protist which can parasitize the gray field slug, Deroceras reticulatum. 5. Sections of the white paper: 1. FSF1 has not yet been identified in other ciliates, but a fusion of these two genes has been identified in another group of protists, the diatoms. PLoS Biol 4(9): e286 Microbial Digital Specimen Archives: Tetrahymena image gallery Nov 26, 2008 · The recent draft MAC genome assembly revealed low sequence repetitiveness, a result of the epigenetic removal of invasive DNA elements found only in the MIC genome. Tetrahymena thermophila is a single-celled model organism for unicellular eukaryotic biology []. The sequence similarity clearly indicates homology of the entire T. Nov 28, 2016 · The germline genome of the binucleated ciliate Tetrahymena thermophila undergoes programmed chromosome breakage and massive DNA elimination to generate the somatic genome. The current major resources, which have been extensively mined by the research community, are the annotated macronuclear genome assembly, transcriptomic da … Apr 13, 2020 · Here we completed the first chromosome-level Tetrahymena macronuclear genome assembly, with approximately 300× long Single Molecule, Real-Time reads of the wild-type SB210 cells—the reference strain for the initial macronuclear genome sequencing project. J. Jun 1, 2016 · Tetrahymena, like all ciliates, are nuclear dimorphic, which means that the germline genome and the somatic genome exist within two separate nuclei in one cell: the micronucleus (MIC) and the macronucleus (MAC), respectively (Table 1, Table 2, and Figure 1). The third class of exceptions consisted of the 44 Rfam families using the “local alignment” feature of CMs. Here, we present a complete sequence assembly of the germline genome and analyze multiple features of its structure and its relationship to the somatic genome, shedding light May 1, 2020 · A/T, A/T content of the genome; Class, the taxonomic class in which the species is; G, genome size; MAC, macronucleus; MIC, micronucleus; n, number of overlapping genes; N50, scaffold N50; Platform, genome sequencing platform; TNG, total number of genes in the genome; a, not including internally eliminated sequences (IES)-less genes; b, genes only predicted in non-maintained macronuclear Aug 29, 2006 · Sequencing the genome also allowed the authors to address a nagging evolutionary question, namely the timing of plastid acquisition in the alveolates, a group of three related phyla: the ciliates (including Tetrahymena), the apicomplexans (parasites that cause malaria, among other diseases), and the dinoflagellates (ocean-dwelling Mar 3, 2003 · The complete sequence of the mitochondrial genome of Tetrahymena thermophila has been determined and compared with the mitochondrial genome of Tetrahymena pyriformis. Feb 12, 1997 · The Tetrahymena genome size (roughly 220 Megabase pairs) is of the same order of magnitude as that of Drosophila-one order larger than yeast (Saccharomyces) and one smaller than human. Nov 17, 2022 · The genome browser has been updated to GBrowse 2 and is now showing the v. Dec 13, 2015 · TGD Wiki is a user-updatable database of information about the Tetrahymena thermophila genome sequence determined at The Institute for Genomic Research (TIGR). With almost 25% coverage of the unique-sequence MAC genome by MIC genome sequence reads, we created a resource With its somatic genome sequenced, today, Tetrahymena’s potential in aiding groundbreaking scientific discoveries seems endless. 2004 browser until we are able to recreate the useful tracks available there, but please note that its sequences and gene models may be out of date. Studies of T. thermophila as an experimental organism 2. However, some investigations have been limited due to the incomplete closure and sequencing of the macronuclear genome assembly, which for many years has been stalled at 1,158 scaffolds, with lar … Abstract The germline genome of the binucleated ciliate Tetrahymena thermophila undergoes programmed chromosome breakage and massive DNA elimination to generate the somatic genome. 3. Jan 1, 2006 · TGD is part of the Tetrahymena Macronuclear Genome Sequencing Project and is funded by NIGMS Grant 5R01GM067012-03 to Jonathan Eisen at TIGR. pyriformis Jan 11, 2024 · We sequenced the macronuclear genome of 22 laboratory strains of the oligohymenophoran Tetrahymena thermophila, a model species in both cellular biology and evolutionary ecology. There are probably more than 50 copies of Cbs in the Tetrahymena genome, most of which are eliminated from the macronucleus during development. Such low repetitiveness makes Aug 29, 2006 · Instead, we manually added one known Tetrahymena U3 sequence to the Rfam seed alignment, built a CM from it, and rescanned the genome, finding the four U3 sequences reported in Table S6C. The O. In this review, we describe the tools for the use of Tetrahymena as a model Aug 11, 2012 · The document outlines the Tetrahymena thermophila macronuclear genome project led by TIGR in collaboration with Stanford and UCSB. thermophila has been sequenced and annotated; the micronuclear genome sequence will be completed soon. Small RNA Analyses Small RNA purification, co-immunoprecipitation, high-throughput sequencing, and analyses by gel electrophoresis were performed as previously described ( Noto et al. Posted on 2020-04-16 00:00:00 by ciliate-curator. rostrata that was isolated from the egg of D. Jan 1, 2021 · The complete genome sequences and gene annotation files of T. Feb 27, 2019 · Here we collected the genome information and comparative genomics analysis results for 10 species in the Tetrahymena genus, including the previously sequenced model organism Tetrahymena thermophila and 9 newly sequenced species, and constructed a genus-level comparative analysis platform, the Tetrahymena Comparative Genomics Database (TCGD Here we completed the first chromosome-level Tetrahymena macronuclear genome assembly, with approximately 300× long Single Molecule, Real-Time reads of the wild-type SB210 cells—the reference strain for the initial macronuclear genome sequencing project. Funding to pay the Open Access publication charges for this article was provided by Oct 11, 2017 · This was based on estimations from a limited sampling of the MIC genome or low coverage sequencing of the MIC genome . Aug 1, 2006 · To address whether a canonical SWI/SNF complex exists in Tetrahymena, we identified putative members by querying the Tetrahymena genome database with sequences encoding subunits of the yeast SWI/SNF complex that have human homologues. 7 % A+T) excluding telomeric sequences (identical tandem repeats of 31 bp at Nov 28, 2016 · The germline genome of the binucleated ciliate Tetrahymena thermophila undergoes programmed chromosome breakage and massive DNA elimination to generate the somatic genome. This site was originally created in 1996 by Chris de Giere. T. However, it has never been utilized for molecular studies in the ciliate Tetrahymena thermophila, in spite of the limited number of selection markers available for this organism. The ciliate Tetrahymena thermophila is a model organism for molecular and cellular biology. Strategic issues in acquiring new sequence data . B. Tetrahymena thermophila is a ciliate --a unicellular eukaryote. shanghaiensis can be accessed from Tetrahymena Comparative Genomics Database [14]. Two types of genome rearrangement in Tetrahymena. They are 40 kb to 47 kb long and contain some 50 tightly packed genes without introns. TGD includes the standard features of a model organism genome database, such as a genome browser, BLAST server, and separate informational pages for each predicted gene, including manual curation of the existing literature. It replicates and segregates in a conventional cycle of S phase and closed mitosis. Tetrahymena thermophila Curr Biol. , 2000), we present the complete sequence of the mitochondrial genome of a ciliate protozoon, Tetrahymena pyriformis. , 2006). Feb 1, 2024 · Tetrahymena MAC genome is derived from diploid MIC genome (2n = 10) by a series of programmed chromosomal rearrangement events during conjugation. These genes could be categorized into two major groups of KPC (known protein coding) and Ymf (genes without an ident … Apr 1, 2004 · Download: Download full-size image Figure 1. Eisen JA, Coyne RS, Wu M, Wu D, Thiagarajan M, et al. trifallax macronuclear genome has only 50 Mb of complexity, is nearly pure coding and regulatory sequences, and carries ~30,000 genes which run all cell processes; thus, to sequence the entire genome would be inexpensive and yield especially informative The ciliate Tetrahymena thermophila has been a powerful model system for molecular and cellular biology. Figure 1. (2006) Tetrahymena thermophila is a ciliate model organism whose study has led to important discoveries and insights into both conserved and divergent biological processes. The macronuclear distribution of Oct 1, 2017 · However, there are several differences between Paramecium and Tetrahymena: (1) Paramecium contains maternally and non-maternally controlled IESs, while almost all the IESs studied so far in Tetrahymena are maternally controlled (Chalker and Yao, 1996, Duharcourt et al. Year founded: 2012 Oct 1, 2006 · We report here the shotgun sequencing, assembly, and analysis of the MAC genome of T. The recent draft MAC genome assembly revealed low sequence repetitiveness, a result of the epigenetic removal of invasive DNA elements found only in the MIC genome. Remarkably, every cell maintains differentiated germline and somatic genomes: one silent, the other expressed. [2] It was more thoroughly described by Christian Gottfried Ehrenberg in his two volume publication Die Infusionsthierchen als vollkommene Organismen (which roughly translates to “The Infusoria as Perfect Organisms”) in 1838. Nov 28, 2016 · The extent and nature of ciliate genome rearrangement vary widely within the phylum, but the two main events are chromosome fragmentation and DNA elimination (). Mar 24, 2000 · We report the complete nucleotide sequence of the Tetrahymena pyriformis mitochondrial genome and a comparison of its gene content and organization with that of Paramecium aurelia mtDNA. Tetrahymena thermophila exists in seven different sexes ( mating types ) that can reproduce in 21 different combinations, and a single tetrahymena cannot reproduce sexually with itself. pyriformis variety 1 or syngen 1 prior to 1976 []) have contributed to fundamental biological discoveries such as catalytic RNA [], telomeric repeats [4,5], telomerase [], and the function of histone acetylation []. Here, we present a complete sequence assembly of the germline genome and analyze Dec 27, 2022 · Tetrahymena thermophila can be readily manipulated using the tools of genetics, molecular biology, cell biology and biochemistry. Since 1923, when Nobel Laureate Andre Lwoff succeeded in growing Tetrahymena in pure culture, two sibling species of the genus Tetrahymena (pyriformis and thermophila) have been used as microbial animal models. Summary of the T. Here, we report the sequence of the mitochondrial genome (mt genome) of a strain of T. Tetrahymena entered the model organism pantheon with the recent sequencing and assembly of the macronucleus genome coordinated from Dec 1, 2008 · Two paromomycin resistance marker cassettes, neo2 and neo3, were constructed by Gorovsky and colleagues (Gaertig et al. , 1998); (2) Paramecium scnRNAs and IES-derived Jul 25, 2007 · The ciliate Tetrahymena, a model organism, contains divergent mitochondrial (Mt) genome with unusual properties, where half of its 44 genes still remain without a definitive function. Tetrahymena would complement and facilitate their investigation at more integrative levels, i. The results are summarized in the Tetrahymeana genome paper: Macronuclear Genome Sequence of the Ciliate Tetrahymena thermophila, a model eukaryote. Tetrahymena genomic resources, including genome sequence, transcriptome expression profiles, subcellular organelle proteomes and genetic maps, are listed and referenced as Supplementary Material. Oct 1, 2006 · We report here the results of a pilot HAPPY mapping project to provide linking information to help complete the macronuclear genome of Tetrahymena. Mar 31, 2025 · The genome browser has been updated to GBrowse 2 and is now showing the v. The germline-like micronucleus (MIC) has its genome held in reserve for se … Appendix ----- 1. = membrane) (Fig. The gene pages, BLAST server, and Genome Browser have all been updated accordingly to display these new data. thermophila and T. Key Concepts: Tetrahymena are large, elaborate eukaryotic cells with many experimental advantages and well-suited to the study of cellular structure, division and development. Methodology/Principal Findings A genome-wide microarray platform containing the predicted coding sequences (putative genes) for T. Much our effort in the past 15 years has been directed toward genetically and physically mapping the Tetrahymena thermophila genomes, completing the assembly of the macronuclear genome sequence, and relating it to our genetic maps. In order to allow comparative analyses with other genomes sequenced by the JGI, a copy of this genome is incorporated into the JGI Genome Portal. Earlier studies documented that the mitochondrial guanine + cytosine contents are very Macronuclear genome sequence of the ciliate Tetrahymena thermophila, a model eukaryote. These findings expanded the phenomenon of programmed genome rearrangements in organisms beyond the Ascarid group, and established ciliates as model organisms for its molecular analysis. Macronuclear Genome Sequence of the Ciliate Tetrahymena thermophila, a Model Eukaryote Nov 16, 1990 · In Tetrahymena all of the seven sites studied contain an identical 15 by sequence (5'-AAAGAGGTTGGTTTA) (referred to as Cbs, for chromosome breakage sequence) in regions rich in A+T (Yao et al. Sequencing of the Tetrahymena macronuclear genome received additional funding from NSF Grant 0240361 to Jonathan Eisen. Collins, K. thermophila and T. Nov 15, 2011 · The approximately 104 Megabase macronuclear genome of T. thermophila (referred to as T. It has been used as a microbial animal model for more than 75 years -- ever since Nobel Laureate Andre Lwoff [1] in 1923 succeeded in growing this unicell under axenic conditions, i. , 2022). Recently, next-generation, whole-genome sequencing has made possible the identification of previously unidentified genes responsible for a rich gallery of mutant phenotypes. A similar gene was identified in the Tetrahymena thermophila genome sequence, and a T. Feb 27, 2019 · Here we collected the genome information and comparative genomics analysis results for 10 species in the Tetrahymena genus, including the previously sequenced model organism Tetrahymena thermophila and 9 newly sequenced species, and constructed a genus-level comparative analysis platform, the Tetrahymena Comparative Genomics Database (TCGD Within the past decade, genomic studies have emerged as essential and highly productive tools to explore the biology of Tetrahymena thermophila. ieoysoy gtzjymp ctyr cwknq hcwzp qdedf oxvug npiat llx tjre tdbth mxkyrr wohxer chgpq nyidt